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JoVE Journal
Developmental Biology

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Kinetic Measurement and Real Time Visualization of Somatic Reprogramming
 

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

Article DOI: 10.3791/54190
July 30th, 2016

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Summary July 30th, 2016

The protocol presented in this study describes methods for the real-time monitoring of reprogramming progression via the kinetic measurement of positive and negative pluripotent stem cell markers using flow cytometry analysis. The protocol also includes the imaging-based assessment of morphology, and marker or reporter expression during iPSC generation.

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