Uma Lakshmipathy

Life Sciences Solutions, Thermo Fisher Scientific

Research Area

Biography

Uma Lakshmipathy, is the Director of R&D in Cell Biology at Thermo Fisher Scientific. She received her doctoral degree in Molecular Biophysics from the Center for Cellular and Molecular Biology in India and postdoctoral experience in DNA double strand break repair from University of Minnesota Medical School. As a junior faculty at the Stem Cell Institute, University of Minnesota, her work involved developing ex vivo gene repair systems that enable correction of single gene mutations in adult stem cells. Her current work is focused on the development of novel products and services related to stem cells. Her work has resulted in the development of novel cell engineering platform, robust somatic reprogramming systems and comprehensive characterization tools. She is currently involved in clinical-grade pluripotent stem cell generation and cell therapy characterization platforms.

JoVE Journal Publications

ArticleTotal : 2	Year
Publication title Cited by 5	2016
Publication title Cited by 1	2020

Other Publications

Article	Year
Efficient transfection of embryonic and adult stem cells. Stem cells (Dayton, Ohio)\| PubMed ID: 15277699	2004
Stem cell plasticity. Blood reviews\| PubMed ID: 15572215	2005
Expression of bacterial endonucleases in Saccharomyces cerevisiae mitochondria. Mitochondrion\| PubMed ID: 16120308	2002
Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research. Stem cells (Dayton, Ohio)\| PubMed ID: 17284651	2007
Creation of engineered human embryonic stem cell lines using phiC31 integrase. Stem cells (Dayton, Ohio)\| PubMed ID: 17962703	2008
Concise review: MicroRNA expression in multipotent mesenchymal stromal cells. Stem cells (Dayton, Ohio)\| PubMed ID: 17991914	2008
MicroRNA expression pattern of undifferentiated and differentiated human embryonic stem cells. Stem cells and development\| PubMed ID: 18004940	2007
PDGF, TGF-beta, and FGF signaling is important for differentiation and growth of mesenchymal stem cells (MSCs): transcriptional profiling can identify markers and signaling pathways importan... Blood\| PubMed ID: 18332228	2008
Micro RNA profiling: an easy and rapid method to screen and characterize stem cell populations. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 18453251	2007
Gene transfer via nucleofection into adult and embryonic stem cells. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 18453252	2007
Differentiating human multipotent mesenchymal stromal cells regulate microRNAs: prediction of microRNA regulation by PDGF during osteogenesis. Experimental hematology\| PubMed ID: 18657893	2008
A single EBV-based vector for stable episomal maintenance and expression of GFP in human embryonic stem cells. Regenerative medicine\| PubMed ID: 19317643	2009
Generation of platform human embryonic stem cell lines that allow efficient targeting at a predetermined genomic location. Stem cells and development\| PubMed ID: 19355838	2009
A simplified culture and polymerase chain reaction identification assay for quality control performance testing of stem cell media products. Cytotherapy\| PubMed ID: 19878062	2009
hESC engineering by integrase-mediated chromosomal targeting. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 19907981	2010
Global Characterization and Genomic Stability of Human MultiStem, A Multipotent Adult Progenitor Cell. Journal of stem cells\| PubMed ID: 20498688	2009
A novel serum-free medium for the expansion of human mesenchymal stem cells. Stem cell research & therapy\| PubMed ID: 20504289	2010
miRNA in pluripotent stem cells. Regenerative medicine\| PubMed ID: 20632858	2010
BacMam-mediated gene delivery into multipotent mesenchymal stromal cells. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 21431539	2011
Chromatin insulator elements block transgene silencing in engineered human embryonic stem cell lines at a defined chromosome 13 locus. Stem cells and development\| PubMed ID: 21699412	2012
Efficient non-viral integration and stable gene expression in multipotent adult progenitor cells. Stem cells international\| PubMed ID: 21977042	2011
Novel live alkaline phosphatase substrate for identification of pluripotent stem cells. Stem cell reviews\| PubMed ID: 22426885	2012
Development and characterization of a clinically compliant xeno-free culture medium in good manufacturing practice for human multipotent mesenchymal stem cells. Stem cells translational medicine\| PubMed ID: 23197667	2012
Stable transfection using episomal vectors to create modified human embryonic stem cells. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 23546763	2013
Advances in genetic modification of pluripotent stem cells. Biotechnology advances\| PubMed ID: 23856320	2013
CD44 is a negative cell surface marker for pluripotent stem cell identification during human fibroblast reprogramming. PloS one\| PubMed ID: 24416407	2014
Characterizing Pluripotent Stem Cells Using the TaqMan® hPSC Scorecard(TM) Panel. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 25138722	2016
Current methods and challenges in the comprehensive characterization of human pluripotent stem cells. Stem cell reviews\| PubMed ID: 25504379	2015
Good Cell Culture Practice for stem cells and stem-cell-derived models. ALTEX\| PubMed ID: 27554434	2017
Generation and comprehensive characterization of induced pluripotent stem cells for translational research. Regenerative medicine\| PubMed ID: 31115261	2019