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Journal
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Developmental Biology
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从人胎盘组织源分离和间充质干扩张/基质细胞
JoVE Journal
Developmental Biology
Author Produced
This content is Free Access.
JoVE Journal
Developmental Biology
Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue
Please note that all translations are automatically generated.
Click here for the English version.
从人胎盘组织源分离和间充质干扩张/基质细胞
DOI:
10.3791/54204-v
•
12:40 min
•
June 06, 2016
•
Rebecca A. Pelekanos
,
Varda S. Sardesai
,
Kathryn Futrega
,
William B. Lott
,
Michael Kuhn
,
Michael R. Doran
3
1
UQ Centre for Clinical Research
,
The University of Queensland
,
2
Translational Research Institute
,
Queensland University of Technology
,
3
Translational Research Institute
,
Mater Medical Research – University of Queensland
Chapters
00:00
Title
01:00
Methodologies
02:20
Introduction
03:12
Tissues to be Harvested
03:53
Preparation of the Placenta and the Placental Anatomy
04:40
Dissection of the Decidua (D) Tissue
05:40
Dissection of the Chorionic Plate (CP) Tissue and Dissection of the Chorionic Villi (CV) Tissue
06:36
Mincing and Enzymatic Digestion of the Placental Tissue
07:25
Collection of Mononuclear Cells and Plating into Flasks
09:33
Post Isolation – Cell Growth and Expansion
09:57
Representative Results: Culture MSC and Assay Cells for MSC Characteristics
12:07
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里我们描述了从人足月胎盘的胎儿和母体组织的解剖方法,随后分离并从这些组织的间充质干细胞/基质细胞(MSC)的扩展。
Tags
Mesenchymal Stem Cells
MSC Isolation
Cell Culture
Fetal-derived Cells
Maternal Cells
Decidua
Chorionic Villi
Chorionic Plate
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