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JoVE Journal
Immunology and Infection

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Femur Window Chamber Model for In Vivo Cell Tracking in the Murine Bone Marrow
 

Femur Window Chamber Model for In Vivo Cell Tracking in the Murine Bone Marrow

Article doi: 10.3791/54205
July 28th, 2016

Summary July 28th, 2016

The protocol describes a novel murine femur window chamber model that can be used to track movement of cells in the femoral bone marrow in vivo. Intravital multiphoton fluorescence microscopy is used to image three components of the femoral bone marrow (vasculature, collagen matrix, and neutrophils) over time.

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