Optical Access
Optical Access - Related Videos
08:31
Three-dimensional Optical-resolution Photoacoustic Microscopy
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Cited by 27 •
2011
Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging technology capable of imaging optical absorption contrasts in vivo with cellular resolution and sensitivity. Here, we provide a visualized instruction on the experimental protocols of OR-PAM, including system configuration, system alignment, typical in vivo experimental procedures, and functional imaging schemes.
14:37
Accessibility to Clinical Care for People who use Wheelchairs
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2023
Source: Yetsa Tuakli-Wosornu1,2, Jaideep Talwalkar1; 1Yale School of Medicine, 2University of Pittsburgh In the United States, 25% of the general population suffers from one or another type of disability. Ambulatory disabilities, or mobility impairments, represent the most common subcategory, comprising 14% of the country's populace. Different mobility-assistive devices, ranging from canes to scooters, enable increased independence and improved quality of life for those suffering from mobility...
09:39
Optical Biosensing
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2023
Optical biosensors utilize light to detect the binding of a target molecule. These sensors can utilize a label molecule, which produces a measurable signal such as fluorescence, or these sensors can be label-free and use the changes in optical properties, such as refractive index, to sense for the binding of the target molecule. This video introduces both label and label-free optical biosensors, demonstrates their use in the laboratory, and shows some applications of the technology.
03:58
Implantation of Cranial Imaging Window in Mouse Model: A Surgical Procedure to Implant Glass-based Coverslip for Stable Optical Access to Regions of Murine Brain
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2023
This video presents a surgical procedure to implant cranial window in a mouse model for stable optical access to different regions of the brain.
11:56
Central Venous Access Device Dressing Change
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2023
Source: Madeline Lassche, MSNEd, RN and Katie Baraki, MSN, RN, College of Nursing, University of Utah, UT Central venous access devices (CVAD), commonly known as central lines or central catheters, are large-bore intravenous (IV) catheters that are introduced into the central circulation. Typically, CVADs terminate in the superior vena cava, just outside of the right atrium of the heart, but they may also terminate in any one of the great veins (i.e., aorta, inferior vena cava, brachiocephalic...
FAQs
These approaches provide a stable or inherently available path for light to reach tissue and for returning signals to be observed. Implanted imaging windows create a prepared viewing route, whereas naturally exposed tissues can be examined without that implantation step. The choice affects how researchers follow cellular events and structures during living-system observation.
Transmitted light can reveal tissue structures and changes in vascular appearance, while fluorescent light makes labeled cells or pathogens detectable against surrounding tissue. Using these signals allows investigators to distinguish moving immune cells, infectious agents, and host structures during the same observation. The resulting images connect cellular behavior with changes in the local tissue environment.
Repeated observation captures change over time rather than only a final state. In infection studies, this can show how immune-cell migration, pathogen distribution, vascular changes, or treatment responses develop during disease progression. Fixed tissue analysis provides endpoint information, but longitudinal imaging can expose the sequence and timing of events that produced that endpoint.
Researchers can combine fluorescent labeling with direct observation of tissue structures and labeled cells or pathogens. This makes it possible to follow the location and movement of immune cells alongside infectious agents while also monitoring vascular changes. In immunology and infection research, the comparison helps relate host defense activity to pathogen behavior and disease progression.
A study first establishes access through an implanted imaging window or an available exposed tissue. Researchers then apply transmitted or fluorescent light to observe labeled cells, pathogens, or tissue structures. Repeated imaging follows selected events over time, allowing investigators to compare immune-cell migration, infection progression, vascular changes, or treatment responses within the same living system.
It is particularly valuable when the research question concerns dynamic interactions rather than a single endpoint. Intravital microscopy can track immune-cell migration, host-pathogen interactions, vascular changes, and responses to treatment. Because the same living system can be observed repeatedly, the approach can improve interpretation of infection progression and immune defense while reducing reliance on separate endpoint samples.