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Biology

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Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture
 

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Article DOI: 10.3791/54207
July 22nd, 2016

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Summary July 22nd, 2016

An RNA pull-down protocol is optimized here for detection of interactions between RNA-binding proteins (RBPs) and noncoding as well as coding RNAs. An RNA fragment from androgen receptor (AR) was used as an example to demonstrate how to retrieve its RBP from lystate of primary brown adipocytes.

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