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Developmental Biology
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结合活体荧光显微镜 (IVFM) 和遗传模型研究造血细胞对骨髓生态位的植入动力学
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches
Please note that all translations are automatically generated.
Click here for the English version.
结合活体荧光显微镜 (IVFM) 和遗传模型研究造血细胞对骨髓生态位的植入动力学
DOI:
10.3791/54253-v
•
11:06 min
•
March 21, 2017
•
Lin Wang*
1
,
Malgorzata M. Kamocka*
2
,
Amy Zollman
,
Nadia Carlesso
1
Wells Center for Pediatric Research, Department of Pediatrics
,
Indiana University School of Medicine
,
2
Indiana Center for Biological Microscopy, Department of Medicine
,
Indiana University School of Medicine
,
3
Department of Pediatrics
,
Indiana University School of Medicine
Chapters
00:05
Title
01:26
Donor Lysozyme Promoter (Lys)-EGFP Mouse Bone Marrow Cell Preparation for Bone Marrow Cell Transplantation (BMT)
03:16
Lys-GFP BMT and Intravital Imaging Preparation
05:23
In Vivo
High Resolution Mouse Calvarium Imaging
07:15
Quantitative Analysis
08:14
Results: Representative Kinetics and Localization of Myeloid Cell Engraftment Following BMT
10:10
Conclusion
Summary
Automatic Translation
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Automatic Translation
活体荧光显微镜 (IVFM) 的颅骨是应用与遗传动物模型, 研究造血细胞归巢和植入的骨髓 (BM) 龛。
Tags
Intravital Fluorescent Microscopy
IVFM
Hematopoietic Cell Engraftment
Bone Marrow Niches
Hematopoietic Cell Regeneration
Cell-to-cell Interactions
Myeloproliferative Disease
Notch Signaling
EGFP Mouse
Bone Marrow Transplant
Cell Isolation
Cell Suspension
Tail Vein Injection
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