Journal
/
Genetics
/
In Vitro Transcription Assays and Their Application in Drug Discovery
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
In Vitro Transcription Assays and Their Application in Drug Discovery

In Vitro Transcription Assays and Their Application in Drug Discovery

DOI:
10.3791/54256-v

•

09:28 min

•

September 20, 2016

•

,
1School of Environmental and Life Sciences,University of Newcastle, 2Department of Applied Biology and Chemical Technology, The State Key Laboratory of Chirosciences,The Hong Kong Polytechnic University

Chapters

  • 00:05Title
  • 00:51Preparation of RNA Sequencing Gel
  • 02:08Transcription Elongation Pausing Assay
  • 04:30RNA Gel Run and Gel Development
  • 06:46Results: NusA Regulates Transcription Pausing
  • 08:34Conclusion

Summary

Automatic Translation

Automatic Translation

In this manuscript, we describe a protocol to functionally examine transcription and the inhibitory activity of antibacterial agents targeting bacterial transcription.

Tags

Keywords: In Vitro Transcription AssayTranscription RegulationTranscription FactorsTranscription InhibitorsMolecular BiologyRNA Sequencing GelRNA PolymeraseSigma 70 FactorOpen ComplexTranscription BufferGTPUTPATPP32-labeled UTP

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/54256?article_type=v

siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts
check_url/54256?article_type=v

Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans
check_url/54256?article_type=v

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
check_url/54256?article_type=v

In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells
check_url/54256?article_type=v

High Fat Diet Feeding and High Throughput Triacylglyceride Assay in Drosophila Melanogaster
check_url/54256?article_type=v

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
check_url/54256?article_type=v

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms
check_url/54256?article_type=v

Novel Sequence Discovery by Subtractive Genomics
check_url/54256?article_type=v

Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models
check_url/54256?article_type=v

Determining the Egg Fertilization Rate of Bemisia tabaci Using a Cytogenetic Technique

Read Article