Radioactive Rna Detection
Radioactive Rna Detection - Related Videos
17:38
Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue
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Cited by 21 •
2012
This protocol is successfully used to quantitatively detect levels and spatial patterns of mRNA expression in multiple tissue types across vertebrate species. The method can detect low abundance transcripts and allows processing of hundreds of slides simultaneously. We present this protocol using expression profiling of avian embryonic brain formation as an example.
03:18
Radioactivity and Nuclear Equations
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2020
Nuclear chemistry is the study of reactions that involve changes in nuclear structure. The nucleus of an atom is composed of protons and, except for hydrogen, neutrons. The number of protons in the nucleus is called the atomic number (Z) of the element, and the sum of the number of protons and the number of neutrons is the mass number (A). Atoms with the same atomic number but different mass numbers are isotopes of the same element. A nuclide of an element has a specific number of protons and...
02:42
RNA CISH Signal Detection: A Technique to Detect Chromogenic Signals During RNA In Situ Hybridization in Intact Tissue Specimens
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2023
This video presents the protocol for signal detection during RNA chromogenic in situ hybridization. This technique can be used to diagnose an active oncogenic infection visually in an intact tissue specimen.
10:34
Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture
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Cited by 19 •
2016
An RNA pull-down protocol is optimized here for detection of interactions between RNA-binding proteins (RBPs) and noncoding as well as coding RNAs. An RNA fragment from androgen receptor (AR) was used as an example to demonstrate how to retrieve its RBP from lystate of primary brown adipocytes.
02:48
Radioactive Decay and Radiometric Dating
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2020
Radioactivity is a spontaneous disintegration of an unstable nuclide and is a random process, as all the nuclei in the sample do not decay simultaneously. The number of disintegrations per unit time is called the activity (A), which is directly proportional to the number of nuclei in the sample. The decay constant (λ) is an average probability of decay per nucleus in unit time. The SI unit for activity is the becquerel, which is one disintegration per second. Another unit of activity is the...
FAQs
Discrimination depends on sequence-specific hybridization between the target RNA and a complementary radioactive probe, or between a radioactive RNA sample and a matching nucleic-acid sequence. Defined hybridization conditions govern whether the interacting strands remain associated. This selectivity allows the radioactive signal to reveal a particular transcript even when many other RNA molecules are present.
Signal patterns can provide information about transcript abundance, transcript size, and differences in RNA processing. A stronger or weaker recorded signal supports comparisons of RNA levels, while bands or mapped signals of different sizes can indicate distinct transcript forms. These measurements help connect gene regulation with the RNA products observed in a sample.
Hybridization conditions determine how effectively the labeled molecule associates with its complementary target sequence. Because the method relies on that association, changes in the defined conditions can affect which sequences are detected and how clearly they are distinguished. Careful control is therefore important when comparing transcript patterns among samples or experimental conditions.
After radioactive labeling and hybridization, the emitted radiation must be recorded to make the molecular pattern measurable. Autoradiography captures the signal as an image, whereas a phosphor-imaging system records the emitted radiation for analysis. Either readout can expose transcript patterns, allowing researchers to assess RNA presence, relative abundance, and size-related differences.
A typical workflow incorporates a radioactive isotope into an RNA sample or complementary nucleic-acid probe, exposes it to the sample under defined hybridization conditions, and records the resulting radiation. The recorded pattern is then examined for target-specific signals and differences among samples. This sequence links molecular recognition to a measurable genetic expression result.
The technique is useful when researchers need to compare RNA patterns across tissues, developmental stages, or experimental conditions. It supports Northern blotting for examining transcript abundance and size, while transcript mapping helps locate or characterize RNA sequence relationships. These applications make the method valuable for investigating gene regulation and RNA processing in genetics.
Different RNA processing outcomes can produce distinguishable transcript patterns when their sizes or mapped sequence relationships differ. By recording radioactive signals from complementary target interactions, researchers can compare these patterns within or between samples. The resulting evidence helps identify changes in transcript forms and relate them to genetic regulation under different tissues, stages, or experimental conditions.