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Bioengineering
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自动化脂双层膜的形成使用聚二甲基硅氧烷薄膜
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film
Please note that all translations are automatically generated.
Click here for the English version.
自动化脂双层膜的形成使用聚二甲基硅氧烷薄膜
DOI:
10.3791/54258-v
•
08:23 min
•
July 10, 2016
•
Sangbaek Choi
3
,
Sunhee Yoon
3
,
Hyunil Ryu
3
,
Sun Min Kim
3,4
,
Tae-Joon Jeon
3,4
1
Department of Biological Engineering
,
Inha University
,
2
Department of Mechanical Engineering
,
Inha University
,
3
Biohybrid Systems Research Center (BSRC)
,
Inha University
,
4
Convergent Research Center for Metabolism and Immunoregulation (CRCMI)
,
Inha University
Chapters
00:05
Title
00:32
Lipid Bilayer Solutions Preparation
01:38
Formation of a Polydimethylsiloxane (PDMS) Thin Film
02:45
Chamber Fabrication and Assembly
03:36
Membrane Formation and Verification
04:47
Electrical Recording of the Lipid Bilayer
05:42
Gramicidin A Ion Channel Incorporation
06:58
Results: Measuring the Incorporation of Gramicidin A into a Lipid Bilayer
07:46
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们展示了一个可存储,运输的脂质双分子层形成系统。的脂质双层膜可以在1小时内超过80%的成功率,当冷冻膜前体达到环境温度下形成。该系统将减少与离子通道相关的繁琐流程和专业知识。
Tags
Lipid Bilayer
Polydimethylsiloxane (PDMS)
Thin Film
Automated Membrane Formation
Nanobiosensor
Membrane-protein Interactions
Buffer Solution
Lipid Solution
Pre-painting
Black Lipid Membrane Chamber
PTFE
CNC Machining
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