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JoVE Journal
Biology

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Genetic and Biochemical Approaches for In Vivo and In Vitro Assessment of Protein Oligomerization
 

Genetic and Biochemical Approaches for In Vivo and In Vitro Assessment of Protein Oligomerization: The Ryanodine Receptor Case Study

Article DOI: 10.3791/54271 12:43 min
July 27th, 2016

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Summary July 27th, 2016

Oligomerization of the ryanodine receptor, a homo-tetrameric ion channel mediating Ca2+ release from intracellular stores, is critical for skeletal and cardiac muscle contraction. Here, we present complementary in vivo and in vitro methods to detect protein self-association and determine homo-oligomer stoichiometry.

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