Hek293 Cells
Hek293 Cells - Related Videos
08:17
Production of High-Yield Adeno Associated Vector Batches Using HEK293 Suspension Cells
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Cited by 3 •
2024
Here, a suspension HEK293 cell-based AAV production protocol is presented, resulting in reduced time and labor needed for vector production using components that are available for research purposes from commercial vendors.
11:42
High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension
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Cited by 76 •
2015
Laboratory-scale production of eukaryotic proteins with appropriate post-translational modification represents a significant barrier. Here is a robust protocol with rapid establishment and turnaround for protein expression using a mammalian expression system. This system supports selective amino acid, selective labeling of proteins and small molecule modulators of glycan composition.
07:51
Inhibitory Synapse Formation in a Co-culture Model Incorporating GABAergic Medium Spiny Neurons and HEK293 Cells Stably Expressing GABAA Receptors
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Cited by 14 •
2014
The molecular mechanisms that co-ordinate the formation of inhibitory GABAergic synapses during ontogeny are largely unknown. To study these processes,we have developed a co-culture model system which incorporates embryonic medium spiny GABAergic neurons cultured together with stably transfected human embryonic kidney 293 (HEK293) cells expressing functional GABAA receptors.
09:40
In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells
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Cited by 2 •
2017
Small ubiquitin-related modifier (SUMO) family proteins are conjugated to the lysine residues of target proteins to regulate various cellular processes. This paper describes a protocol for the detection of retinoblastoma (Rb) protein SUMOylation under endogenous and exogenous conditions in human cells.
09:12
Optimal Lentivirus Production and Cell Culture Conditions Necessary to Successfully Transduce Primary Human Bronchial Epithelial Cells
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Cited by 13 •
2016
Primary human bronchial epithelial cells are difficult to transduce. This protocol describes the production of lentiviruses and their concentration as well as the optimal culture conditions necessary to achieve highly efficient transductions in these cells throughout differentiation to a pseudostratified epithelium.
FAQs
The adenovirus 5 DNA transformation established the cellular background in which these cells can be maintained as an immortalized research line. That property supports repeated experiments rather than a limited observation window. In bioengineering, researchers can therefore compare molecular designs, expression outcomes, or production strategies using a consistently available human-cell platform.
Efficient transfection allows researchers to introduce genetic material into HEK293 cells and examine the resulting expression of designed genes. This helps determine whether an engineered construct functions as intended before considering a larger biotechnology application. The same feature supports recombinant protein production when the introduced design directs synthesis of the desired product.
Growth behavior and adaptable culture conditions influence how practical an experiment is. Because HEK293 cells grow readily in culture, investigators can maintain them while testing genetic systems or production designs under selected conditions. Their consistent growth helps reduce variation associated with maintaining the cellular platform, making comparisons among constructs or experiments more straightforward.
A typical workflow begins by growing the cells in culture, introducing a genetic construct through transfection, and then examining expression or collecting the resulting product. The endpoint depends on the project: researchers may evaluate gene function, assess an engineered system, produce recombinant protein, or generate material for viral-vector work.
For gene-function studies, researchers introduce a designed genetic system and examine how its expression supports or changes the intended experiment. For recombinant protein production, the introduced construct is used to direct synthesis of a protein product. This range makes the same cell platform useful for both molecular evaluation and biotechnology development.
HEK293 cells provide a platform for producing viral vectors used in gene-delivery research. Their ability to receive introduced genes and support high expression connects genetic design with vector-production work. In bioengineering, this application allows researchers to develop or evaluate delivery-related systems while using a human-cell-based production platform.