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Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells
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Developmental Biology
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JoVE Journal Developmental Biology
Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells

Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells

DOI:
10.3791/54277-v

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08:57 min

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July 25, 2016

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,
1Department of Developmental Pathology,Institute of Pathology, University of Bonn Medical School

Chapters

  • 00:05Title
  • 00:40293T Cell Lentiviral Vector Production
  • 03:06Fibroblast to Induced Trophoblast Stem Cell (iTSC) Conversion
  • 05:10Individual iTSC Line Isolation (Transdifferentiation Day 21-28)
  • 07:02Results: Representative Morphological Changes During MEF to iTSC Conversion
  • 07:56Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a protocol for the direct conversion of murine embryonic fibroblasts into fully functional and stable trophoblast stem cells by ten day over-expression of Tfap2c, Gata3, Eomes and Ets2.

Tags

Keywords: Murine Embryonic FibroblastsTrophoblast Stem CellsInduced Trophoblast Stem CellsITSCsTransfectionLentiviral Vector293T CellsVirus ProductionCell CulturePoly-L-lysineChloroquineHEPES Buffered Saline

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