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Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix
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Bioengineering
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JoVE Journal Bioengineering
Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix

Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix

DOI:
10.3791/54283-v

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08:49 min

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July 10, 2016

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,
1Chemical and Biological Engineering,Princeton University, 2Molecular Biology,Princeton University

Chapters

  • 00:05Title
  • 00:49Preparation of Elastomeric Stamps for 3D Micropatterning
  • 02:48Preparation of 3D Epithelial Tissues
  • 06:58Results: 3D Culture System Provides Insight to Growth Factor Mediated Branching Morphogenesis
  • 07:58Conclusion

Summary

Automatic Translation

Automatic Translation

This manuscript describes a soft lithography-based technique to engineer uniform arrays of three-dimensional (3D) epithelial tissues of defined geometry surrounded by extracellular matrix. This method is amenable to a wide variety of cell types and experimental contexts and allows for high-throughput screening of identical replicates.

Tags

Extracellular MatrixBranching MorphogenesisCollective Cell BehaviorPhotolithographyPDMSMoldStamp

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