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High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits

High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits

DOI:
10.3791/54286-v

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06:51 min

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September 20, 2016

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, ,
1Department for Plant and Environmental Sciences,University of Copenhagen, 2School of Agriculture, Food and Rural Development,Newcastle University

Chapters

  • 00:05Title
  • 00:40Chromogenic Assay with CPH Substrates, Detection, and Quantification
  • 02:41Chromogenic Assay with Insoluble Chromogenic Biomass Substrates, Detection, and Quantification
  • 04:15Results: Enzymatic Degradation of Chromogenic Polymer Hydrogel Substrates
  • 06:02Conclusion

Summary

Automatic Translation

Automatic Translation

A high-throughput assay for enzyme screening is described. This multiplexed ready-to-use assay kit comprises of pre-chosen Chromogenic Polymer Hydrogel (CPH) substrates and complex Insoluble Chromogenic Biomass (ICB) substrates. Target enzymes are polysaccharide degrading endo-enzymes and proteases.

Tags

Keywords: High-throughput ScreeningCarbohydrate-degrading EnzymesNovel Insoluble Chromogenic Substrate Assay KitsEnzyme DiscoveryBiomass DegradationChromogenic SubstratesEndo-cellulaseICB-wheat StrawAbsorbancePlate Reader

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