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Chapters
- 00:05Title
- 00:45Preparing Giant Unilamellar Vesicles (GUVs): Part 1
- 03:25Preparing GUVs: Part 2, and Harvesting the In Vitro Translation Reaction
- 05:47Harvesting the GUVs and Binding Assay
- 06:43Results: HIV-1 Gag Binding to GUVs
- 07:32Conclusion
We illustrate here an in vitro membrane binding assay in which interactions between HIV-1 Gag and lipid membranes are visually analyzed using YFP-tagged Gag synthesized in a wheat germ-based in vitro translation system and GUVs prepared by an electroformation technique.
