Murine Experimental Model of Original Tumor Development and Peritoneal Metastasis via Orthotopic Inoculation with Ovarian Carcinoma Cells
December 9th, 2016
To clarify the multistep process of peritoneal dissemination of ovarian carcinoma, the current study presents a murine experimental model of original tumor development and peritoneal metastasis via orthotopic inoculation with these tumor cells.
The overall goal of this procedure is to observe the development of original and metastatic tumor formation in epithelial ovarian carcinoma.This method can help answer key questions in the gynecologic oncological field, such as understanding the tumor biology or peritoneal defemenation.The main advantage of this technique is that orospatic inoculation of human epithelial ovarian carcinoma, or EOC cells, is performed through a retroperitoneal approach grown with a dorsal flank.To prepare a human ovarian clear cell carcinoma cell line, plate and maintain ES2 cells in RPMI-1640 medium with 10%FBS and PenStrep at 37 degrees Celsius, and 5%CO2.In the logarithmic growth phase, remove the old medium from the culture and use three to four millimeters of PBS to wash the cells once.Next, add one millimeter of trypsin/EDTA and rock the vessel to cover the entire monolayer.Remove and discard the trypsin solution and return the cells to the incubator for three to five minutes.After examining the cells under a microscope to ensure the cells have detached and are floating, add five millimeters of fresh serum-containing growth medium to inactivate the trypsin and re-suspend the cells before transferring them to a 15-millimeter conical tube.Centrifuge the suspension at 300 times G and room temperature
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Overview
This study presents a murine experimental model to elucidate the multistep process of peritoneal dissemination of ovarian carcinoma. The model involves the orthotopic inoculation of tumor cells to study original tumor development and subsequent peritoneal metastasis.
Key Study Components
Area of Science
- Oncology
- Experimental Models
- Metastasis Research
Background
- Ovarian carcinoma is known for its complex metastatic behavior.
- Understanding peritoneal dissemination is crucial for improving treatment strategies.
- Murine models are commonly used to study cancer progression.
- This study aims to provide insights into the mechanisms of metastasis.
Purpose of Study
- To clarify the mechanisms of peritoneal dissemination in ovarian carcinoma.
- To develop a reliable murine model for studying tumor behavior.
- To investigate the role of orthotopic inoculation in metastasis.
Methods Used
- Orthotopic inoculation of tumor cells in mice.
- Monitoring tumor development and metastasis.
- Utilization of imaging techniques to assess tumor spread.
- Analysis of tumor microenvironment interactions.
Main Results
- Successful establishment of a murine model for ovarian carcinoma.
- Observation of peritoneal metastasis following orthotopic inoculation.
- Insights into the tumor microenvironment's role in metastasis.
- Identification of potential therapeutic targets for intervention.
Conclusions
- The murine model effectively simulates peritoneal dissemination.
- Findings contribute to understanding ovarian carcinoma metastasis.
- Future studies may focus on therapeutic strategies based on these insights.
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Chapters in this video
0:05
Title
0:46
Preparation of Cell Suspensions
3:12
Orthotopic Inoculation with Cell Suspensions
5:12
Post-surgical Treatment and Analysis of Tumors and Metastases
5:59
Results: Peritoneal Metastasis via Orthotopic Inoculation of Ovarian Carcinoma Cells
6:48
Conclusion
