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External Excitation of Neurons Using Electric and Magnetic Fields in One- and Two-dimensional Cultures
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JoVE Journal Neuroscience
External Excitation of Neurons Using Electric and Magnetic Fields in One- and Two-dimensional Cultures

External Excitation of Neurons Using Electric and Magnetic Fields in One- and Two-dimensional Cultures

DOI:
10.3791/54357-v

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08:32 min

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May 07, 2017

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, , , ,
1Laboratory of Genetics,The Salk Institute for Biological Studies, 2Department of Physics and SEAS,Harvard University, 3Department of Physics of Complex Systems,Weizmann Institute of Science

Chapters

  • 00:05Title
  • 01:31Preparation of Coverslips for 1D Cultures
  • 03:03Electric Stimulation of Cultures
  • 04:49Magnetic Stimulation of Cultures
  • 06:09Results: The Response of Individual Cells to Electric Field and that of the Ring Cultures to Magnetic Field
  • 07:23Conclusion

Summary

Automatic Translation

Automatic Translation

Neuronal cultures are a good model for studying emerging brain stimulation techniques via their effect on single neurons or a population of neurons. Presented here are different methods for stimulation of patterned neuronal cultures by an electric field produced directly by bath electrodes or induced by a time-varying magnetic field.

Tags

Keywords: Neuronal CulturesElectric StimulationMagnetic StimulationOne-dimensional CulturesTwo-dimensional CulturesNeural ExcitationChronaxieRheobasePatterned Neuronal NetworksElectric FieldsMagnetic FieldsBrain DisordersCell CultureSputteringBiorejecting LayerChromeGoldPen PlotterEtching NeedleRubber Sheet

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