Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Reliable and High Efficiency Extraction of…
JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content.

JoVE Journal Immunology and Infection
Reliable and High Efficiency Extraction of Kidney Immune Cells
Measuring Influenza Neuraminidase Inhibition Antibody Titers by Enzyme-linked Lectin Assay
Journal (Immunology and Infection)
Measuring Influenza Neuraminidase Inhibition Antibody Titers by Enzyme-linked Lectin Assay
Analysis of Microglia and Monocyte-derived Macrophages from the Central Nervous System by Flow Cytometry
Journal (Immunology and Infection)
Analysis of Microglia and Monocyte-derived Macrophages from the Central Nervous System by Flow Cytometry
 

Reliable and High Efficiency Extraction of Kidney Immune Cells

Article DOI: 10.3791/54368-v • 09:15 min • August 19th, 2016
August 19th, 2016 •
1, 2, 3, 3
1Division of Nephrology, Department of Medicine, University of Missouri-Columbia and Harry S Truman Memorial Veteran's Hospital, 2Division of Biomedical Sciences, Department of Medicine, University of Missouri-Columbia and Harry S Truman Memorial Veteran's Hospital, 3Division of Endocrinology, Department of Medicine, University of Missouri-Columbia and Harry S Truman Memorial Veteran's Hospital

Chapters

Summary
Automatic Translation

Techniques that are reliable and efficient for the isolation of kidney immune cells are needed for downstream applications. This requires surface antibody labeling of a small number of kidney immune cells. Herein, we describe a concise method for isolation of kidney immune cells that seemingly achieves this goal.

Tags

Kidney Immune Cells Cell Isolation Flow Cytometry Collagenase Digestion Experimental Analysis Kidney Immunobiology Cytokine Secretion Chemokine Secretion Kidney Disease Immune Cell Isolation Multicolor Flow Cytometry Kidney Immunobiology Field Liver Cells Brain Cells Research Specialist Anesthetized Mouse Abdominal Cavity
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter