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JoVE Journal
Biology

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Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
 

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

Article doi: 10.3791/54434
August 21st, 2016 Usage Statistics

Summary August 21st, 2016

We describe here a system utilizing a site-specific, reversible in vivo protein block to stall and collapse replication forks in Escherichia coli. The establishment of the replication block is evaluated by fluorescence microscopy and neutral-neutral 2-dimensional agarose gel electrophoresis is used to visualize replication intermediates.

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