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Deferred Growth Inhibition Assay to Quantify the Effect of Bacteria-derived Antimicrobials on Competition
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Biology
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Deferred Growth Inhibition Assay to Quantify the Effect of Bacteria-derived Antimicrobials on Competition

Deferred Growth Inhibition Assay to Quantify the Effect of Bacteria-derived Antimicrobials on Competition

DOI:
10.3791/54437-v

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07:42 min

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September 03, 2016

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, , ,
1Institute of Integrative Biology,University of Liverpool

Chapters

  • 00:05Title
  • 00:45Preparing Agar Plates, Sterile Broth, and Sterile Vapoizer Bottles
  • 02:19Prepare Bacterial Overnight Cultures and Spot Culture of the Inhibitor-producing Isolate
  • 03:34Preparing Spray Inoculum of Test Competitor Strains and Deferred Growth Inhibition Assay
  • 04:48Results: Antimicrobial-derived Deferred Growth Inhibition
  • 06:16Conclusion

Summary

Automatic Translation

Automatic Translation

The deferred growth inhibition assay can be used to assess the competition effect by one bacterial isolate on another. Inhibition is quantified by measuring the zone of clearing around the inhibitor-producing isolate, or qualitatively assessed by determining the visible extent of inhibition.

Tags

Keywords: Deferred Growth Inhibition AssayBacterial CompetitionAntimicrobial EffectsBacterial CommunitiesCompetitive ExclusionBrain Heart Infusion AgarBHI BrothVaporizer BottlesOvernight CultureCompetitor StrainInhibitor Strain

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