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Protective Efficacy and Pulmonary Immune Response Following Subcutaneous and Intranasal BCG Administration in Mice
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JoVE Journal Immunology and Infection
Protective Efficacy and Pulmonary Immune Response Following Subcutaneous and Intranasal BCG Administration in Mice

Protective Efficacy and Pulmonary Immune Response Following Subcutaneous and Intranasal BCG Administration in Mice

DOI:
10.3791/54440-v

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06:32 min

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September 19, 2016

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, , ,
1Department of Microbiology,University of Zaragoza

Chapters

  • 00:05Title
  • 00:55Mouse BCG Vaccination and Mycobacterium tuberculosis Challenge
  • 02:18Bronchoalveolar Lavage (BAL) Sample Acquisition
  • 04:11Results: Representative Vaccine-specific Pulmonary Immune Response Analyses
  • 05:46Conclusion

Summary

Automatic Translation

Automatic Translation

We herein detail the methodology followed to compare protective efficacy and lung immune response induced by intranasal and subcutaneous immunization with BCG in mouse model. Our results show the benefits of pulmonary vaccination and suggest a role for IL17-mediated response in vaccine-induced protection.

Tags

Keywords: BCG VaccineTuberculosisProtective EfficacyPulmonary Immune ResponseSubcutaneous AdministrationIntranasal AdministrationIntranasal ChallengeBronchoalveolar LavageLung Immune Response

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