Enumeration of Neural Stem Cells Using Clonal Assays

Gunaseelan Narayanan; Yuan Hong Yu; Muly Tham; Hui Theng Gan; Srinivas Ramasamy; Shvetha Sankaran; Srivats Hariharan; Sohail Ahmed

doi:10.3791/54456

Journal

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Neuroscience

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Conteggio di cellule staminali neurali L'utilizzo clonali Assays

Enumeration of Neural Stem Cells Using Clonal Assays

JoVE Journal
Neuroscience

This content is Free Access.

JoVE Journal Neuroscience

Enumeration of Neural Stem Cells Using Clonal Assays

Conteggio di cellule staminali neurali L'utilizzo clonali Assays

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8,257 Views

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10:32 min

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October 04, 2016

DOI:

10.3791/54456-v

DOI:

10.3791/54456-v

•

10:32 min

October 04, 2016

•

8239 Views

Gunaseelan Narayanan¹, Yuan Hong Yu¹, Muly Tham¹, Hui Theng Gan¹, Srinivas Ramasamy¹, Shvetha Sankaran¹, Srivats Hariharan¹, Sohail Ahmed¹

¹Neural Stem Cell Laboratory, Institute of Medical Biology,Agency for Science, Technology and Research (A*STAR)

Summary

Le cellule staminali neurali (NSC) si riferiscono alle cellule che possono auto-rinnovarsi e di differenziarsi in tre linee neurali. Qui, descriviamo un protocollo per determinare la frequenza NSC in una data popolazione di cellule utilizzando neurosfere formazione e la differenziazione in condizioni clonali.