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成像G蛋白偶联受体介导的趋化和信令活动在中性粒细胞样细胞HL60
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Imaging G Protein-coupled Receptor-mediated Chemotaxis and its Signaling Events in Neutrophil-like HL60 Cells
DOI:

08:24 min

September 14, 2016

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Chapters

  • 00:05Title
  • 01:08Cell Mobility Analysis Device Assembly
  • 03:11Cell Mobility Analysis
  • 06:00Results: Representative Cell Mobility Device Chemotaxis Analysis
  • 07:15Conclusion

Summary

Automatic Translation

视觉趋化实验是为了更好地了解如何真核细胞趋化控制介导的定向细胞迁移是必不可少的。在这里,我们描述了详细的方法:1)实时,多趋化实验的高分辨率监测,和2)同时可视化趋化梯度和信号中的中性粒细胞样HL60细胞事件的时空动力学。

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