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Journal / Genetics / Bitki patojeni olarak genetik manipülasyonu
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Genetics

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JoVE Journal Genetics
Bitki patojeni olarak genetik manipülasyonu
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Bitki patojeni olarak genetik manipülasyonu

Article DOI: 10.3791/54522-v • 11:42 min • September 30th, 2016
September 30th, 2016 •
*1,2, *1, 3, 3, 1,2, 1,2,4
1Institute for Microbiology, Heinrich-Heine University Düsseldorf, 2Bioeconomy Science Center (BioSC), 3Department of Genetics, Institute of Applied Biosciences, Karlsruhe Institute of Technology, 4Cluster of Excellence in Plant Sciences (CEPLAS), Heinrich-Heine University Düsseldorf
* These authors contributed equally

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Genetik Ustilago Maydis'in karalamak mantar işlemek için Biz sağlam bir gen değiştirme stratejisini açıklar. Bu protokol enfeksiyon fenotipleri araştırmak için silme mutantlarını üretmek açıklar. Bir floresan protein etiketi kodlayan bir sekansı ilave olarak, örneğin, arzu edilen herhangi bir şekilde, genleri değiştirme uzatılabilir.

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Genetik Sayı 115, Genetik mühendisliği silme mutant genetik mutant fenotip homolog rekombinasyon model rastık mantarı bitki patojeni gerilme doğrulama ters
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