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Biochemistry

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Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo
 

Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo

Article doi: 10.3791/54527
October 23rd, 2016

Summary October 23rd, 2016

This study describes biophysical, biochemical and molecular techniques to characterize the chaperone activity of Escherichia coli HdeB under acidic pH conditions. These methods have been successfully applied for other acid-protective chaperones such as HdeA and can be modified to work for other chaperones and stress conditions.

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