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In Vivo Gene Transfer to Schwann Cells in the Rodent Sciatic Nerve by Electroporation
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Neuroscience
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JoVE Journal Neuroscience
In Vivo Gene Transfer to Schwann Cells in the Rodent Sciatic Nerve by Electroporation

In Vivo Gene Transfer to Schwann Cells in the Rodent Sciatic Nerve by Electroporation

DOI:
10.3791/54567-v

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05:40 min

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September 08, 2016

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1Department of Pharmacology, Graduate School of Medicine,The University of Tokyo, 2Laboratory for Cell Polarity Regulation, Quantitative Biology Center,RIKEN

Chapters

  • 00:05Title
  • 00:45Animal Surgery, DNA Injection, and Electroporation
  • 03:18Post-electroporation
  • 03:55Results: Representative Results on Transfected Sciatic Nerves
  • 05:17Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present an in vivo technique for gene transfer to Schwann cells (SCs) in the rodent sciatic nerve. This simple technique is useful for investigating signaling mechanisms involved in the development and maintenance of myelinating SCs.

Tags

Keywords: In Vivo Gene TransferSchwann CellsRodent Sciatic NerveElectroporationMyelinationDemyelinationPeripheral Nervous SystemAnesthesiaSciatic Nerve ExposureMicro-pipette InjectionElectroporation PulseWound Closure

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