Journal
/
Developmental Biology
/
The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

DOI:
10.3791/54598-v

•

08:24 min

•

February 28, 2017

•

, , , , , ,
1Stem Cell and Regenerative Medicine Laboratory,Fondazione Istituto di Ricerca Pediatrica Citta della Speranza, 2Lunenfeld-Tanenbaum Research Institute,Mount Sinai Hospital, 3Stem Cells and Regenerative Medicine Section, Developmental Biology and Cancer Programme,UCL Institute of Child Health and Great Ormond Street Hospital

Chapters

  • 00:05Title
  • 00:58Mouse Amniotic Fluid (AF) Cell Isolation
  • 03:24Transfection of Mouse AF Cells
  • 04:24Induced Pluripotent Stem (iPS)-AF Cell Genreation and Culture
  • 06:32Results: Reprogramming of Mouse Amniotic Fluid Cells
  • 07:50Conclusion

Summary

Automatic Translation

Automatic Translation

In this study, we generate induced pluripotent stem cells from mouse amniotic fluid cells, using a non-viral-based transposon system.

Tags

Keywords: Mouse Amniotic Fluid CellsPluripotent Stem CellsTransposon SystemReprogrammingFetal TherapyCell IsolationCell CultureMEF Feeder Cells

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/54598?article_type=v

Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle
check_url/54598?article_type=v

Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids
check_url/54598?article_type=v

Feeder-free Derivation of Melanocytes from Human Pluripotent Stem Cells
check_url/54598?article_type=v

Differentiation of Atrial Cardiomyocytes from Pluripotent Stem Cells Using the BMP Antagonist Grem2
check_url/54598?article_type=v

Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol
check_url/54598?article_type=v

Induced Pluripotent Stem Cell Generation from Blood Cells Using Sendai Virus and Centrifugation
check_url/54598?article_type=v

Chondrogenic Pellet Formation from Cord Blood-derived Induced Pluripotent Stem Cells
check_url/54598?article_type=v

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton
check_url/54598?article_type=v

Semi-automated Production of Hepatocyte Like Cells from Pluripotent Stem Cells
check_url/54598?article_type=v

Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells

Read Article