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A Fluorescence-based Assay of Phospholipid Scramblase Activity
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Biochemistry
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JoVE Journal Biochemistry
A Fluorescence-based Assay of Phospholipid Scramblase Activity

A Fluorescence-based Assay of Phospholipid Scramblase Activity

DOI:
10.3791/54635-v

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09:52 min

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September 20, 2016

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,
1Department of Biochemistry,Weill Cornell Medical College

Chapters

  • 00:05Title
  • 00:45Preparation of Liposomes and Proteoliposomes
  • 02:12Quantification of Phospholipids
  • 03:37Reconstitution of Opsin
  • 06:16Scramblase Activity Assay
  • 07:29Results: Reconstitution of Opsin into LUVs to Characterize Its Scramblase Activity
  • 09:09Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a fluorescence-based assay to measure phospholipid scrambling in large unilamellar liposomes reconstituted with opsin.

Tags

Keywords: Phospholipid Scramblase ActivityFluorescence-based AssayG Protein Coupled ReceptorLipid BilayerPOPCPOPGLarge Unilamellar VesiclesExtrusionPerchloric AcidAmmonium Molybdate

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