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JoVE Journal
Biochemistry

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Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
 

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase

Article DOI: 10.3791/54652
November 23rd, 2016

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Summary November 23rd, 2016

Here, we describe the preparation and use of an activity-based probe (ARN14686, undec-10-ynyl-N-[(3S)-2-oxoazetidin-3-yl]carbamate) that allows for the detection and quantification of the active form of the proinflammatory enzyme N-acylethanolamine acid amidase (NAAA), both in vitro and ex vivo.

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