Microglia as a Surrogate Biosensor to Determine Nanoparticle Neurotoxicity

Cayla M. Duffy; Shihab Ahmed; Ce Yuan; Vijayakumar Mavanji; Joshua P. Nixon; Tammy Butterick

doi:10.3791/54662

Journal

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Neuroscience

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Microglia as a Surrogate Biosensor to Determine Nanoparticle Neurotoxicity

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Microglia as a Surrogate Biosensor to Determine Nanoparticle Neurotoxicity

6,928 Views

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08:37 min

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October 25, 2016

DOI:

10.3791/54662-v

DOI:

10.3791/54662-v

•

08:37 min

October 25, 2016

•

6919 Views

Cayla M. Duffy^1,2, Shihab Ahmed¹, Ce Yuan^1,2,3, Vijayakumar Mavanji¹, Joshua P. Nixon^1,2, Tammy Butterick^1,2,4

¹Minneapolis Veterans Affairs Health Care System ²Department of Food Science and Nutrition,University of Minnesota ³Biomedical Informatics and Computational Biology Program,University of Minnesota ⁴Minnesota Obesity Center,University of Minnesota

Summary

Microglia (immune cells of the brain), are used as a surrogate biosensor to determine how nanoparticles influence neurotoxicity. We describe a series of experiments designed to assay microglial response to nanoparticles and exposure of hypothalamic neurons to supernatant from activated microglia to determine neurotoxicity.