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Microglia as a Surrogate Biosensor to Determine Nanoparticle Neurotoxicity
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Neuroscience
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JoVE Journal Neuroscience
Microglia as a Surrogate Biosensor to Determine Nanoparticle Neurotoxicity

Microglia as a Surrogate Biosensor to Determine Nanoparticle Neurotoxicity

DOI:
10.3791/54662-v

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08:37 min

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October 25, 2016

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, , , , ,
1Minneapolis Veterans Affairs Health Care System, 2Department of Food Science and Nutrition,University of Minnesota, 3Biomedical Informatics and Computational Biology Program,University of Minnesota, 4Minnesota Obesity Center,University of Minnesota

Chapters

  • 00:05Title
  • 01:12Culturing Microglial Cells
  • 03:47Measuring Cytokine Release from Microglial Cells
  • 05:42Using Cultured Hypothalamic Cells for a Toxicity Assay
  • 06:57Results: Cytokine Release from Microglial after AgNP Exposure
  • 07:54Conclusion

Summary

Automatic Translation

Automatic Translation

Microglia (immune cells of the brain), are used as a surrogate biosensor to determine how nanoparticles influence neurotoxicity. We describe a series of experiments designed to assay microglial response to nanoparticles and exposure of hypothalamic neurons to supernatant from activated microglia to determine neurotoxicity.

Tags

Keywords: MicrogliaBiosensorNanoparticleNeurotoxicityNeuroscienceIn VitroCell CultureBV2 CellsSilver NanoparticlesConditioned MediaFiltrationCell CountingCell SeedingCell StarvationCell Activation

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