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Lateral Chronic Cranial Window Preparation Enables In Vivo Observation Following Distal Middle Cerebral Artery Occlusion in Mice
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JoVE Journal Neuroscience
Lateral Chronic Cranial Window Preparation Enables In Vivo Observation Following Distal Middle Cerebral Artery Occlusion in Mice

Lateral Chronic Cranial Window Preparation Enables In Vivo Observation Following Distal Middle Cerebral Artery Occlusion in Mice

DOI:
10.3791/54701-v

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08:41 min

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December 29, 2016

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, , , ,
1Department of Neurosurgery and Center for Stroke-research Berlin (CSB),Charité-Universitätsmedizin, 2Department of Physics,University of California San Diego

Chapters

  • 00:05Title
  • 00:43Lateral Chronic Cranial Window Preparation
  • 03:40Intravital Microscopy
  • 04:20Distal Middle Cerebral Artery Occlusion (MCAo)
  • 07:04Results: Lateral Chronic Cranial Window with Distal MCAo
  • 08:02Conclusion

Summary

Automatic Translation

Automatic Translation

Surgical occlusion of a distal middle cerebral artery branch (MCAo) is a frequently used model in experimental stroke research. This manuscript describes the basic technique of permanent MCAo, combined with the insertion of a lateral cranial window, which offers the opportunity for longitudinal intravital microscopy in mice.

Tags

Keywords: Lateral Chronic Cranial WindowMiddle Cerebral Artery OcclusionIntravital MicroscopyCerebellar IschemiaIn Vivo ImagingStrokeAnesthesiaCraniotomyDental GlueCover GlassSuturesAnalgesia

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