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LED Thermo Flow — Combining Optogenetics with Flow Cytometry
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LED Thermo Flow — Combining Optogenetics with Flow Cytometry

LED Thermo Flow — Combining Optogenetics with Flow Cytometry

DOI:
10.3791/54707-v

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05:49 min

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December 30, 2016

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, , ,
1Max-Planck Institute for Immunobiology und Epigenetics, 2Spemann Graduate School of Biology and Medicine (SGBM),University of Freiburg, 3Centre for Biological Signaling Studies, BIOSS,University of Freiburg, 4Albert-Ludwigs-Universität, 5Institute for Biology III (Mol. Immunology),Albert-Ludwigs-Universität

Chapters

  • 00:05Title
  • 00:33Estimation of Required Light Intensity
  • 02:09Measuring the Kinetics of an Optogenetic Tool
  • 03:28Results: Photoswitching of the Dronpa-Linker-Dronpa Protein
  • 04:57Conclusion

Summary

Automatic Translation

Automatic Translation

Optically controlled substances are powerful tools to study signaling pathways. To expand the spectrum of possible experiments, we developed a device for studying optically controlled substances in real time using flow cytometry: the LED Thermo Flow.

Tags

Keywords: LED Thermo FlowOptogeneticsFlow CytometryCell SignalingOptically Controlled SubstancesDronpaPhotoswitchingRamos B Lymphocytes400 Nm LED500 Nm LEDGlass FACS TubesFluorescence Intensity

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