Journal
/
Immunology and Infection
/
A Murine Model of Group B Streptococcus Vaginal Colonization
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
A Murine Model of Group B Streptococcus Vaginal Colonization

A Murine Model of Group B Streptococcus Vaginal Colonization

DOI:
10.3791/54708-v

•

10:19 min

•

November 16, 2016

•

,
1Department of Pediatrics, Division of Host-Microbe Systems & Therapeutics,University of California San Diego School of Medicine, 2Department of Biology and Center for Microbial Sciences,San Diego State University

Chapters

  • 00:05Title
  • 00:46Beta-Estradiol Intraperitoneal Injection
  • 01:56Vaginal Group B Streptococcus (GBS) Inoculation
  • 04:08Vaginal Lumen Swabbing for GBS Load Quantification
  • 05:17Vaginal Lavage Fluid Collection and Tissue Dissection and Homogenization
  • 07:52Results: Representative Murine GBS Vaginal Colonization Analyses
  • 09:34Conclusion

Summary

Automatic Translation

Automatic Translation

The purpose of this protocol is to imitate human group B Streptococcus (GBS) vaginal colonization in a murine model. This method may be used to investigate host immune responses and bacterial factors contributing to GBS vaginal persistence, as well as to test therapeutic strategies.

Tags

Keywords: Murine ModelGroup B StreptococcusVaginal ColonizationBeta-EstradiolTodd Hewitt BrothCFUInoculumGBS StrainPeritoneal CavityPBS

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/54708?article_type=v

Murine Model of CD40-activation of B cells
check_url/54708?article_type=v

Induction and Assessment of Class Switch Recombination in Purified Murine B Cells
check_url/54708?article_type=v

Using Bioluminescent Imaging to Investigate Synergism Between Streptococcus pneumoniae and Influenza A Virus in Infant Mice
check_url/54708?article_type=v

Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy
check_url/54708?article_type=v

Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of Candida vaginitis
check_url/54708?article_type=v

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
check_url/54708?article_type=v

Characterization of Inflammatory Responses During Intranasal Colonization with Streptococcus pneumoniae
check_url/54708?article_type=v

Non-invasive Imaging of the Innate Immune Response in a Zebrafish Larval Model of Streptococcus iniae Infection
check_url/54708?article_type=v

Analysis of Shear Flow-induced Migration of Murine Marginal Zone B Cells In Vitro
check_url/54708?article_type=v

Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis

Read Article