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Visualization of IL-22-expressing Lymphocytes Using Reporter Mice
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Immunology and Infection
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JoVE Journal Immunology and Infection
Visualization of IL-22-expressing Lymphocytes Using Reporter Mice

Visualization of IL-22-expressing Lymphocytes Using Reporter Mice

DOI:
10.3791/54710-v

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10:30 min

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January 25, 2017

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, , , ,
1Laboratory of Molecular Immunoregulation, Cancer and Inflammation Program, Center for Cancer Research,National Cancer Institute, National Institutes of Health

Chapters

  • 00:05Title
  • 01:49Bacterial Artificial Chromosome (BAC) Recombineering of IL-22-tdTomato Vector Generation
  • 03:22Spleen, Thymus, Lymph Node, and Peyer’s Patch Single Cell Suspension Generation
  • 04:48Intestinal Intraepithelial Lymphocyte and Lamina Propria Cell Isolation
  • 06:40IL-22-tdTomato Colitis Expression
  • 07:57Results: Representative Visualization and Localization of IL-22 Receptors
  • 09:31Conclusion

Summary

Automatic Translation

Automatic Translation

We describe here a transgenic reporter mouse model to visualize the IL-22-producing cells inside different mouse tissues. This method can be used to track the location of other cytokines or secretary proteins in the mouse.

Tags

Keywords: IL-22LymphocytesReporter MiceCytokinesInflammatory Bowel DiseaseBACPCRBacterial TransformationBacterial Culture

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