Induction of Ischemic Stroke and Ischemia-reperfusion in Mice Using the Middle Artery Occlusion Technique and Visualization of Infarct Area

Luc Bertrand; Levi Dygert; Michal Toborek

doi:10.3791/54805

Induction of Ischemic Stroke and Ischemia-reperfusion in Mice Using the Middle Artery Occlusion T...

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Induction of Ischemic Stroke and Ischemia-reperfusion in Mice Using the Middle Artery Occlusion Technique and Visualization of Infarct Area

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09:08 min

February 2, 2017

DOI: 10.3791/54805-v

Luc Bertrand^1,2, Levi Dygert², Michal Toborek^1,3

¹Department of Biochemistry and Molecular Biology, Miller School of Medicine,University of Miami, ²Miller School of Medicine,University of Miami, ³Jerzy Kukuczka Academy of Physical Education

Overview

This article describes a surgical procedure to induce ischemic stroke in a mouse model by occluding the middle cerebral artery. The method allows for the external cerebral artery to remain intact, facilitating direct treatment infusion into the affected hemisphere.

Key Study Components

Area of Science

Neuroscience
Stroke Research
Animal Models

Background

Ischemic stroke is a critical area of study in neuroscience.
Understanding treatment impacts on stroke severity is essential.
Maintaining the external cerebral artery during occlusion is advantageous.
This model can be used for both permanent occlusion and temporary ischemia.

Purpose of Study

To reliably induce ischemic stroke in mice.
To explore the effects of various treatments on stroke outcomes.
To provide a model for studying stroke mechanisms and therapies.

Methods Used

Mouse anesthesia with isoflurane and oxygen.
Placement of a silicone-coated suture for artery occlusion.
Monitoring of respiratory rate and reflexes during surgery.
Post-stroke treatment infusion through the external cerebral artery.

Main Results

The procedure successfully induced ischemic stroke.
External cerebral artery remained intact for treatment infusion.
Potential for further research into stroke therapies.
Method demonstrated reliability and reproducibility.

Conclusions

This model is a valuable tool for stroke research.
It allows for direct treatment application post-occlusion.
Further studies can build on this foundational technique.

Frequently Asked Questions

What is the main goal of this surgical procedure?

The main goal is to reliably induce ischemic stroke while keeping the external cerebral artery intact for treatment infusion.

How is the mouse prepared for the surgery?

The mouse is anesthetized with isoflurane and placed on a prepared surgery surface.

What are the advantages of this stroke model?

The model allows for direct infusion of treatments into the affected hemisphere after stroke induction.

Can this method induce both permanent and temporary ischemia?

Yes, the protocol can be applied for both permanent occlusion and temporary ischemia followed by reperfusion.

What monitoring is required during the procedure?

The respiratory rate and reflex responses of the mouse must be monitored to ensure proper anesthesia.

What impact does this research have on stroke treatment?

This research can help answer key questions regarding the effectiveness of treatments on stroke severity.

We describe a mouse model of stroke induced by the occlusion of the middle cerebral artery using a silicone coated suture. The protocol can be applied to induce permanent occlusion or a temporary ischemia, followed by reperfusion.

The overall goal of this surgical procedure is to officially and reliably induce Ischemic stroke while leaving the external cerebral artery intact. This method can help answer key questions in the field of stroke research, such as the impact of treatment on stroke severity. The main advantage of this technique is that following stroke induction, the external cerebral artery remains available to infuse treatment directly into the affected hemisphere.

Place a mouse anesthetized with isoflurane onto the prepared surgery surface and fit a nose cone to administer isoflurane mixed with oxygen. Ensure that the respiratory rate is around one to two respirations per second without gasping. In addition, ensure that the animal does not exhibit a reaction to whisker stimulation or the pedal reflex.

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