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Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans
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JoVE Journal Genetics
Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans

Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans

DOI:
10.3791/54810-v

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04:51 min

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November 14, 2016

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1Division of Biological Sciences, Section of Neurobiology,University of California in San Diego and Howard Hughes Medical Institute, 2Department of Cellular and Molecular Medicine,University of California in San Diego School of Medicine and Howard Hughes Medical Institute

Chapters

  • 00:05Title
  • 00:46Assembling the LED Illuminator
  • 01:52Blue Light Treatment
  • 03:46Results: Screen Mutation Rate
  • 04:27Conclusion

Summary

Automatic Translation

Automatic Translation

Genetically-encoded histone-miniSOG induces genome-wide heritable mutations in a blue light-dependent manner. This mutagenesis method is simple, fast, free of toxic chemicals, and well-suited for forward genetic screening and transgene integration.

Tags

Keywords: OptogeneticRandom MutagenesisHistone-miniSOGC. ElegansLEDReactive Oxygen SpeciesROS-generating ProteinsMutagenesis ProtocolBlue LightDigital Function GeneratorPhotometerCopper ChlorideSine WaveYoung Adult Hermaphrodites

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