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JoVE Journal
Immunology and Infection

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Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Article DOI: 10.3791/54832 09:42 min January 2nd, 2017
January 2nd, 2017



An optimized protocol is presented for the generation of monoclonal antibodies based on the hybridoma technology. Mice were immunized with an immunoconjugate. Spleen cells were fused by PEG and an electric impulse with immortal myeloma cells. Antibody-producing hybridoma cells were selected by HAT and antigen-specific ELISA screening.



Hybridoma Technology Monoclonal Antibodies Antibody Production Biotechnological Production Specific Binders Detection And Visualization ELISA Flow Cytometry Magnetic Cell Sorting Myeloma Cells Murine SP2/0 Cells Culture Medium Incubation Feeder Cells NMRI Mouse RPMI Medium
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