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JoVE Journal
Immunology and Infection

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Generation of Murine Monoclonal Antibodies by Hybridoma Technology
 

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Article DOI: 10.3791/54832
January 2nd, 2017

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Summary January 2nd, 2017

An optimized protocol is presented for the generation of monoclonal antibodies based on the hybridoma technology. Mice were immunized with an immunoconjugate. Spleen cells were fused by PEG and an electric impulse with immortal myeloma cells. Antibody-producing hybridoma cells were selected by HAT and antigen-specific ELISA screening.

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