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Evaluation of Bioenergetic Function in Cerebral Vascular Endothelial Cells
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Evaluation of Bioenergetic Function in Cerebral Vascular Endothelial Cells

Evaluation of Bioenergetic Function in Cerebral Vascular Endothelial Cells

DOI:
10.3791/54847-v

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06:15 min

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November 19, 2016

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, , ,
1Department of Physiology and Pharmacology,West Virginia University, 2Mitochondrial Evaluation Core,West Virginia University, 3Experimental Stroke Core,West Virginia University, 4Center for Basic and Translational Stroke Research,West Virginia University

Chapters

  • 00:05Title
  • 00:36Cerebral Vascular Endothelial (CVE) Cell Culture and Passage
  • 01:48Mitochondrial Functional Assessment
  • 03:27Results: Representative CVE Bioenergetic Function Analyses
  • 05:10Conclusion

Summary

Automatic Translation

Automatic Translation

Endothelial cell mitochondria are critical to maintain blood-brain-barrier integrity. We introduce a protocol to measure bioenergetic function in cerebral vascular endothelial cells.

Tags

Keywords: Mitochondrial FunctionNeurological DisordersBlood-brain BarrierCerebral Vascular Endothelial CellsExtracellular FluxCell CultureCell MediumTrypsin EDTACell SeedingCell AttachmentTreatmentMitochondrial Functional AssessmentExtracellular Flux Sensor CartridgeExtracellular Flux Assay MediumBioanalyzer

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