Journal
/
Neuroscience
/
Using Linear Agarose Channels to Study Drosophila Larval Crawling Behavior
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Using Linear Agarose Channels to Study Drosophila Larval Crawling Behavior

Using Linear Agarose Channels to Study Drosophila Larval Crawling Behavior

DOI:
10.3791/54892-v

•

06:08 min

•

November 26, 2016

•

,
1Committee on Development, Regeneration, and Stem Cell Biology,University of Chicago, 2Department of Molecular Genetics and Cell Biology,University of Chicago

Chapters

  • 00:05Title
  • 00:52Collecting Larvae
  • 02:05Preparing the Channels
  • 03:01Loading Larvae into the Channels
  • 04:40Results: Larval Movement in Linear Channels
  • 05:36Conclusion

Summary

Automatic Translation

Automatic Translation

The Drosophila larva is a powerful model system to study neural control of behavior. This publication describes the use of linear agarose channels to elicit sustained bouts of linear crawling and methods to quantify the dynamics of larval structures during repetitive crawling behavior.

Tags

Keywords: Drosophila Larval CrawlingLinear Agarose ChannelsNeuro-ethologyDevelopmental BiologyMotor PatternsNeural CircuitsLarval BehaviorPDMS Casting MoldAgarose ChannelsLarval Health Assessment

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/54892?article_type=v

Single Sensillum Recordings in the Insects Drosophila melanogaster and Anopheles gambiae
check_url/54892?article_type=v

Laser Capture Microdissection of Drosophila Peripheral Neurons
check_url/54892?article_type=v

Visualization of Larval Segmental Nerves in 3rd Instar Drosophila Larval Preparations
check_url/54892?article_type=v

Morphological Analysis of Drosophila Larval Peripheral Sensory Neuron Dendrites and Axons Using Genetic Mosaics
check_url/54892?article_type=v

Methods to Assay Drosophila Behavior
check_url/54892?article_type=v

Live Imaging of Drosophila Larval Neuroblasts
check_url/54892?article_type=v

A Simple Way to Measure Alterations in Reward-seeking Behavior Using Drosophila melanogaster
check_url/54892?article_type=v

Quantification of Drosophila Grooming Behavior
check_url/54892?article_type=v

Examining Monosynaptic Connections in Drosophila Using Tetrodotoxin Resistant Sodium Channels
check_url/54892?article_type=v

Tracking Drosophila Larval Behavior in Response to Optogenetic Stimulation of Olfactory Neurons

Read Article