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Journal / Developmental Biology / Rapid neuronal Differensiering av Induced…
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
Rapid neuronal Differensiering av Induced Pluripotent stamceller for måling Network Aktivitet på Micro-elektrode Arrays
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Rapid neuronal Differensiering av Induced Pluripotent stamceller for måling Network Aktivitet på Micro-elektrode Arrays

Article DOI: 10.3791/54900-v • 09:20 min • January 8th, 2017
January 8th, 2017 •
*1,2, *3, 2,3, 3, 1,2, 1,2, 1,2, 2,3,4, 1,2,3
1Department of Cognitive Neurosciences, Radboudumc, 2Donders Institute for Brain, Cognition and Behaviour, Radboud University, 3Department of Human Genetics, Radboudumc, 4Department of Molecular Developmental Biology, Radboud University
* These authors contributed equally

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Vi endre og implementere en tidligere utgitt protokoll som beskriver den raske, reproduserbar og effektiv differensiering av menneskeskapte Pluripotent stamceller (hiPSCs) til eksitatoriske kortikale nevroner 12. Nærmere bestemt tillater vår modifikasjon for kontroll av neuronal celletetthet og bruk av mikroelektrodegrupper for å måle elektrofysiologiske egenskaper på nettverksnivå.

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Developmental Biology induserte pluripotente stamceller neuronal differensiering mikro-elektrode matriser lentiviral transduksjon astrocyte isolasjon nevronale nettverk
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