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Journal / Developmental Biology / Romlig og tidsmessig analyse av Active ERK i
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Developmental Biology

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JoVE Journal Developmental Biology
Romlig og tidsmessig analyse av Active ERK i
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Romlig og tidsmessig analyse av Active ERK i

Article DOI: 10.3791/54901-v • 08:40 min • November 29th, 2016
November 29th, 2016 •
1, 1,2
1Program in Developmental Biology, Baylor College of Medicine, 2Department of Genetics, UT MD Anderson Cancer Center

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Vi presenterer en immunfluorescens bildebasert metode for romlig og tidsmessig lokalisering av aktive ERK i dissekert C. elegans gonade. Protokollen er beskrevet her kan tilpasses for visualisering av en hvilken som helst signalering eller strukturelt protein i C. elegans gonade, gitt en passende antistoff reagens er tilgjengelig.

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Developmental Biology RAS-ERK, Germline, immunfluorescens signalanlegg proteiner
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