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November 29, 2016
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Summary
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Se presenta un método basado en la formación de imágenes de inmunofluorescencia para la localización espacial y temporal de ERK activa en la C. diseccionado gónadas elegans. El protocolo aquí descrito se puede adaptar para la visualización de cualquier señalización o la proteína estructural en la C. elegans gónada, proporciona un reactivo de anticuerpo adecuado está disponible.
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Cite this Article
Gervaise, A. L., Arur, S. Spatial and Temporal Analysis of Active ERK in the C. elegans Germline. J. Vis. Exp. (117), e54901, doi:10.3791/54901 (2016).
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