A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:02Bacterial Cultures and FAME Preparation and Analysis
- 02:35GC-MS Conditions
- 03:30Picolinyl Ester Preparation from FAME
- 04:44DMOX Preparation from FAME
- 05:47Results: Identification of Fatty Acids in Bacillus cereus
- 07:36Conclusion
We propose a protocol to identify fatty acids without the need to purify them. It combines information on the retention times with the mass spectra of three types of fatty acid derivatives: fatty acid methyl esters (FAMEs), 4,4-dimethyl oxazoline derivatives (DMOX), and 3-pyridylcarbinyl esters (picolinyl).
