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Factores artificiales de ingeniería para manipular específicamente empalme alternativo en células humanas
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Factores artificiales de ingeniería para manipular específicamente empalme alternativo en células humanas

Article DOI: 10.3791/54967-v • 10:06 min • April 26th, 2017
April 26th, 2017 •
*1, *1, 2, 1, 1, 1, 1
1Key Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences (SIBS), 2Institute of Cancer Stem Cell, Second Affiliated Hospital, Cancer Center, Dalian Medical University
* These authors contributed equally

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Este informe describe un método bioingeniería para diseñar y construir nuevos factores de empalme artificiales (ASFS) que modulan específicamente el empalme de genes diana en células de mamífero. Este método se puede ampliar para diseñar diversos factores artificiales para manipular otros aspectos del metabolismo del RNA.

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Genética factores de unión de ARN artificiales ingeniería de proteínas factores de empalme corte y empalme alternativo de andamio PUF proteínas de unión de ARN cáncer
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