Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death

Snezana Vujicic; Lanfei Feng; Angelika Antoni; Joyce Rauch; Jerrold S. Levine

doi:10.3791/54980

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Идентификация внутриклеточных сигнальных событий, наведенных в жизнеспособные клетки при взаимодействии с соседними сотами Проходят апоптотической гибели клеток

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death

JoVE Journal
Biology

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JoVE Journal Biology

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death

Идентификация внутриклеточных сигнальных событий, наведенных в жизнеспособные клетки при взаимодействии с соседними сотами Проходят апоптотической гибели клеток

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8,537 Views

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09:18 min

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December 27, 2016

DOI:

10.3791/54980-v

DOI:

10.3791/54980-v

•

09:18 min

December 27, 2016

•

3 Views

Snezana Vujicic*^1,2, Lanfei Feng*^1,2, Angelika Antoni³, Joyce Rauch⁴, Jerrold S. Levine^1,2,5

¹Section of Nephrology, Department of Medicine,University of Illinois at Chicago, ²Section of Nephrology, Department of Medicine,Jesse Brown Veterans Affairs Medical Center, ³Department of Biology,Kutztown University of Pennsylvania, ⁴Division of Rheumatology, Department of Medicine,Research Institute of the McGill University Health Centre, ⁵Department of Microbiology & Immunology,University of Illinois at Chicago

Transcript

Summary

Here, we present a protocol for the determination of intracellular signaling events induced in viable cells by physical interaction with adjacent dead or dying cells. The protocol focuses on signaling events induced by receptor-mediated recognition of the dead cells, as opposed to their phagocytic uptake or release of soluble mediators.