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Production of Replication-Defective Retrovirus by Transient Transfection of 293T cells
 

Production of Replication-Defective Retrovirus by Transient Transfection of 293T cells

Article DOI: 10.3791/550
December 4th, 2007

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Summary December 4th, 2007

This technique demonstrates an efficient way to prepare replication-defective retroviral stocks encoding a human oncogene, and subsequently used for induction of myeloproliferative disease in the mouse model.

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