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Inoculating Anopheles gambiae Mosquitoes with Beads to Induce and Measure the Melanization Immune Response
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Immunology and Infection
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JoVE Journal Immunology and Infection
Inoculating Anopheles gambiae Mosquitoes with Beads to Induce and Measure the Melanization Immune Response

Inoculating Anopheles gambiae Mosquitoes with Beads to Induce and Measure the Melanization Immune Response

DOI:
10.3791/55013-v

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08:24 min

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January 12, 2017

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, , ,
1Laboratoire d’écologie et d’épidémioloigie parasitaire, Institut de biologie,Université de Neuchâtel, 2Merkle Lab, Department of Entomology,Pennsylvania State University

Chapters

  • 00:05Title
  • 00:44Preparation of Saline Solution and Bead Selection with Capillary
  • 02:21Mosquito Handling and Inoculation
  • 03:52Mosquito Dissection and Bead Photography
  • 04:59Picture Analysis: Bead Melanization
  • 06:05Results: Inoculating Anopheles gambiae Mosquitoes with Beads to Induce and Measure the Melanization Immune Response
  • 07:39Conclusion

Summary

Automatic Translation

Automatic Translation

Through inoculation with beads, the described technique enables the stimulation of the mosquito melanization response in the hemolymph circulating system. The amount of melanin covering the beads can be measured after dissection as a measure of the immune response.

Tags

Keywords: Anopheles GambiaeMelanizationImmune ResponseInoculationBeadsCapillary InjectionEcological ImmunologyMethyl GreenMosquito RearingInsect Dissection

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