Methyl Green Solution
Methyl Green Solution - Related Videos
05:59
Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos
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Cited by 24 •
2015
A method for fluorescent staining of fixed biological material with the specific DNA label methyl green is described. Methyl green is used in a diluted aqueous solution and is very resistant to photobleaching. Its far-red emission allows for deep specimen imaging, making it particularly adequate for whole embryos.
08:47
DNA Methylation Analysis
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2023
Methylation at CpG dinucleotides is a chemical modification of DNA hypothesized to play important roles in regulating gene expression. In particular, the methylation of clusters of methylation sites, called “CpG islands”, near promoters and other gene regulatory elements may contribute to the stable silencing of genes, for example, during epigenetic processes such as genomic imprinting and X-chromosome inactivation. At the same time, aberrant CpG methylation has been shown to be associated with...
12:34
DNA Methylation: Bisulphite Modification and Analysis
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Cited by 75 •
2011
The gold standard for DNA methylation analysis is genomic sequencing of bisulphite converted DNA. This method takes advantage of the increased sensitivity of cytosine compared with 5-methylcytosine (5-MeC) to bisulphite deamination under acidic conditions. Unmethylated cytosines can be distinguished from methylated cytosines after PCR amplification of the target genomic DNA.
21:24
Methylated DNA Immunoprecipitation
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Cited by 43 •
2009
This video demonstrates the protocol for methylated DNA immunoprecipitation (MeDIP). MeDIP is a two day procedure that selectively extracts methylated DNA fragments from a genomic DNA sample using antibodies with specificity for 5 -methylcytosine (anti-5 mC).
08:40
Methyl-binding DNA capture Sequencing for Patient Tissues
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Cited by 1 •
2016
Here we present a protocol to investigate genome wide DNA methylation in large scale clinical patient screening studies using the Methyl-Binding DNA Capture sequencing (MBDCap-seq or MBD-seq) technology and the subsequent bioinformatics analysis pipeline.
FAQs
Its staining behavior depends on the interaction between the cationic dye and acidic nucleic acids. Under suitable staining conditions, methyl green shows a strong preference for DNA, causing nuclei with abundant DNA to appear green. This chemical selectivity makes nuclear boundaries and overall nuclear distribution easier to evaluate in microscopic specimens.
Combining methyl green with pyronin adds an RNA-oriented contrast to the DNA-focused nuclear signal. In this paired approach, green-stained nuclei can be considered alongside RNA-rich cytoplasmic regions. That separation provides context for examining cellular activation and proliferation, especially when immune cells or infected tissues show altered cellular organization.
Changes in nuclear morphology can provide structural context for immune or infectious processes. Examining nuclear appearance and organization within tissue sections helps relate cellular changes to the surrounding specimen rather than viewing isolated signals alone. The stain therefore supports interpretation of tissue responses associated with immune activity or pathogen-associated changes.
To use methyl green solution, researchers stain cells or tissue sections and then examine the specimen microscopically. The resulting green nuclear signal can be evaluated for nuclear morphology and cellular organization. When the sample is studied in immunology or infection research, observations are interpreted alongside the broader tissue changes visible in the specimen.
In immunology, the stain can help place cellular changes within their tissue setting by showing nuclear organization and, when paired with pyronin, RNA-rich cytoplasmic regions. In infection research, the same readout supports examination of pathogen-associated tissue responses. Its value is contextual because it links cellular and nuclear features to specimen-level changes.
Suitable staining conditions are important because the expected DNA preference and green nuclear signal depend on how the dye associates with acidic nucleic acids in the specimen. If those conditions are appropriate, researchers can more reliably examine nuclear morphology and organization. This consistency supports clearer comparison of cellular and tissue changes across microscopic observations.