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Immunology and Infection

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在从幼稚的CD4 ⁺ T细胞^的人CD4 ⁺ FOXP3 ⁺诱导调节性T细胞（iTregs的）使用含有TGF-β-协议的体外分化

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Immunology and Infection

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JoVE Journal Immunology and Infection

In Vitro Differentiation of Human CD4⁺FOXP3⁺ Induced Regulatory T Cells (iTregs) from Naïve CD4⁺ T Cells Using a TGF-β-containing Protocol

Please note that all translations are automatically generated. Click here for the English version.

在从幼稚的CD4 ⁺ T细胞^的人CD4 ⁺ FOXP3 ⁺诱导调节性T细胞（iTregs的）使用含有TGF-β-协议的体外分化

DOI:

10.3791/55015-v

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08:20 min

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December 30, 2016

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Angelika Schmidt, Szabolcs Éliás*¹, Rubin N. Joshi*¹, Jesper Tegnér

¹Unit of Computational Medicine, Center for Molecular Medicine, Department of Medicine Solna,Karolinska Institutet, Karolinska University Hospital, & Science for Life Laboratory

Chapters

00:05Title
01:03Peripheral Blood T Cell Isolation
03:43Treg Induction, Plating and Monitoring, and Staining
05:52Results: Representative CD4⁺FOXP3⁺ iTreg Analyses
07:25Conclusion

Summary

Automatic Translation

本协议描述从幼稚CD4 ⁺ T细胞在体外可重复产生和人为调节性T细胞（iTregs的）的表型。不同的协议FOXP3诱导允许与各自的协议获取特定表型iTreg的研究。

Tags

Keywords: In Vitro Differentiation Human CD4+FOXP3+Induced Regulatory T Cells ITregs Naive CD4+ T Cells TGF-Î² Phenotype T Cell Immunology PBMC Magnetic Bead Isolation Biotin Antibody Anti-biotin Microbeads LS Column

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